Effectiveness of Sludge Microbial Consortia in the Bioremediation of Detergent-Containing Launderette Wastes

  • Yan Ramona 1 School of Biology, Faculty of Mathematics and Sciences, Udayana University, 2 Integrated Laboratory for Biosciences and Biotechnology, Udayana University, Jl. Raya Kampus UNUD, Bukit Jimbaran, Kuta Selatan, Badung-Bali-80361
  • Esti Arisetya Dewi School of Biology, Faculty of Mathematics and Sciences, Udayana University, Jl. Raya Kampus UNUD, Bukit Jimbaran, Kuta Selatan, Badung-Bali-80361 Indonesia
  • Yenni Ciawi Environmental Engineering Department, Faculty of Engineering, Udayana University, Jl. Raya Kampus UNUD, Bukit Jimbaran, Kuta Selatan, Badung-Bali-80361 Indonesia
  • I Wayan Budiarsa Suyasa School of Chemistry, Faculty of Mathematics and Sciences, Udayana University Jl. Raya Kampus UNUD, Bukit Jimbaran, Kuta Selatan, Badung-Bali-80361
Keywords: Bali, Bioremediation, COD, Detergent, Loundry, Microbial, Consortia

Abstract

This project investigated the potential of microbial consortia obtained from several sources to reduce or eliminate detergent concentration in the wastes of launderette businesses found in the capital city of Bali. Before being used as active starters, the consortium of microbiota contained in the 3 waste sources was enriched in a medium containing (g/l); 1 glucose, 0.05 K2HPO4, 0.05 KH2PO4, 0.05 (NH4)2[Fe(SO4)2].6H2O, 0.01 MgSO4, 0.01 yeast extract, 1800 mL distilled water, and 200 mL of wastewater of launderette industries. An important parameter used to indicate microbial growth was the level of the volatile solid substrate (VSS) in the enrichment medium. The microbial culture that showed the highest VSS only was further investigated in the main experiments by observing its effectiveness in reducing detergent linear alkyl-benzenesulfonates (LAS) and the chemical oxygen demand (COD) of launderette wastes. In this project, microbial consortia developed from detergent-containing launderette wastes were the most active starter to decrease the COD and LAS detergent content following 7 days of exposure. This starter (microbial consortia developed from this launderette waste) showed the highest value of VSS when compared to those collected from other sources, and this was reached on day 4. In the main experiment, 85.5% and 91.9% reduction of LAS and the COD of the wastes, respectively, were observed following exposure of the launderette waste with this culture starter. This indicated that these microbial consortia have the possibility to be developed as a potential starter in larger scales of detergent bioremediation.

References

Adesoye AM, Olayinka K, Olukomaiya OO, and Iwuchukwu PO. (2014). The removal of phosphates from laundry wastewater using alum and ferrous sulphate as coa-gulants. International Journal of Innovation and Scientific Research. 8(2): 256-260. Doi: http://www.ijisr.issr-journals.org/.

Department of Health of Republic of Indonesia. (1993). Pemeriksaan air buangan dan air kolam renang cetakan ke-2. Depkes RI Pusat Labortorium Kesehatan. Jakarta.

Embrandiri A, Kiyasudeen SK, Rupani PF, Ibrahim MH. (2016). Environmental xenobiotics and its effects on natural ecosystem. In: Singh, A., Prasad, S., Singh, R. (eds) Plant Responses to Xenobiotics. Springer, Singapore. https://doi.org/10.1007/978-981-10-2860-1_1.

Greiner JV, Glonek T. (2021). Intracellular ATP Concentration and implication for cellular evolution. Biology. 10(11):1166. https://doi.org/10.3390/biology10111166

Ginting, P. (2007). Sistem Pengolahan Lingkungan dan Limbah Industri. Yrama Widya. Bandung.

Halling PJ. (2020). Thermodynamic favorability of end products of anaerobic glucose metabolism. ACS Omega 5: 15843−15849. Doi:https://dx.doi.org/10.1021/acsomega.0c00790.

Himeoka HandKaneko K. (2017). Theory for transitions between exponential and stationary Phases:Universal Laws for Lag Time. PHYS. REV. X 7: 021049(16). Doi:10.1103/PhysRevX.7.021049.

Ibayati Y. (2003). Pintar Biologi 3. Ganeca. Jakarta

Keputusan Menteri Negara Lingkungan Hidup. (1995) Keputusan Menteri Negara Lingkungan Hidup No. KEP-51/MENLH/10 /1995 tentang Baku Mutu Limbah Cair Bagi Kegiatan Industri.

Krishnamurthi VR, Niyonshuti II, Chen J, Wang Y. (2021). A newanalysis method for evaluating bacterial growth with microplate readers. PLoS ONE 16(1): e0245205. https://doi.org/10.1371/journal.pone.0245205

Kundu S, Coumar MV, Rajendiran S, Ajay, and Rao AS. (2015). Phosphates from detergents and eutrophication of surface water ecosystem in India. Current Science, 108, 1320-1325.

Clesceri LS, Greenberg AE, and Eaton AD. (1998). Standard methods for the examination of water and waste water 20th Edition. APHA AWWA WEF. Washington DC.

Osadebe AU, Onyiliogwu CA, Okpokwasili GC. (2018). Biode-gradation of anionic surfactants from oilfield detergents in aquatic systems. Universal Journal of Microbiology Research 6(1): 7-14. Doi: 10.13189/ujmr.2018.060102.

Rinawati, Hidayat D, Suprianto R, Dewi P S. (2016). Penentuan kandu-ngan zat padat (total dissolved solid dan total suspended solid) di perairan teluk Lampung. Analit: Analytical and Environmental Chemistry. 1(01): 36 – 45.

Stanaszek-Tomal E. (2020). Environ-mental factors causing the development of microorganisms on the surfaces of national cultural monuments made of mineral building materials—Review. Coatings; 10(12):1203. Doi: 10.3390/coatings10121203

Statistical Bureau of Bali Province. (2021). Provinsi Bali Dalam Angka. BPS Provinsi Bali/BPS – Statistics of Bali Province. Denpasar-Bali.

Toledo NAB, Contreras CH, Corredor CAA, and Perez CAR. (2021). Effect of biodegradable detergents on water quality.Nat. Volatiles & Essent. Oils 8(5): 12080-12095.

Waluyo L. (2004). Mikrobiologi Umum. Universitas Muhamma-diyah Malang Press. Malang.

Wisjnuprapto. (1995). Pengantar bioproses. Jurusan Teknik Ling-kungan FTSP ITB. Bandung.

Wulandari WA and Soedjono ES. (2021). Penurunan COD dan deterjen pada saluran kalidami kota Surabaya dengan oksidator H2O2 dan KMnO4. Jurnal Teknik ITS. 6(2): F445 – F450.

Yunusa S, Mohammed-Dabo IA, and Ahmed AS. (2017). Formulation of critical micellar concentration of non-phosphate detergents by rurface tension measurement. Nigerian Journal of Basic and Applied Science. 25(1): 94-100. Doi: Doi: 10.4314/njbas.v25i1.13.

Zairinayati ZR and Shatriadi H. (2019). Biodegradasi fosfat pada limbah laundry menggunakan bakteri consorsium pelarut fosfat. Jurnal Kesehatan Lingkungan Indonesia 18(1):57-61. Doi: 10.14710/jkli.18.1.57-61

Zavala MAL and Estrada EE. (2016). The contribution of the type of detergent to domestic laundry graywater composition and its effect on treatment performance. Water. 8, 214. Doi: 10.3390/w8050214.

Published
2022-06-22
How to Cite
Ramona, Y., Dewi, E. A., Ciawi, Y., & Suyasa, I. W. B. (2022). Effectiveness of Sludge Microbial Consortia in the Bioremediation of Detergent-Containing Launderette Wastes. Cassowary, 5(2), 103-111. https://doi.org/10.30862/casssowary.cs.v5.i2.162
Section
Articles