Identifikasi molekuler bakteri indigenous penghasil enzim amilase dari pati sagu asal Poom Kepulauan Yapen
Molecular identification of indigenous bacteria that produce amylase enzyme from sago starch from Poom, Yapen Islands
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Abstract
ABSTRACT: This study aimed to identify indigenous bacterial isolates producing Amylase ezyme from sago starch from Poom, Yapen Islands, using the 16S rDNA sequence molecular method. The isolates identified were IA4 and IA8, which exhibited the highest values of assimilase enzyme activity. Bacteria were identified based on DNA amplification using Polymerase Chain Reaction (PCR) with primers 27F and 1492R. Based on the results of molecular identification, the similarity index of Isolate IA4 and Bacillus cereus D21 is 97.80%, while isolate IA8 had a similarity index of 99.79% with Alcaligenes faecalis Y5. Therefore, the amylolytic bacterial isolates were named Bacillus cereus strain IA4 and Alcaligenes faecalis strain IA8.
References
Asmuruf, F., Wanma J F., Rumatora A. 2018. Budidaya Pemanfaatan Sagu (Metroxylon sp.) Oleh Sub-Etnis Ayamaru di Kampung Sembaro Distrik Ayamaru Selatan. Jurnal Kehutanan Papuasia, 4 (2): 114-127.
Cappucinno, J. G., Sherman N. 2014. Micro-biology: A Laboratory Manual. Tenth Edi-tion. Pearson Education Limited. London.
Devi, L. S., Khaund P., Joshi S. R. 2010. Thermostable α-amilase from natural vari-ants of Bacillus spp. prevalent in eastern Hilmayan range. African Journal of Mi-crobiology Research, 2(4): 573-579.
Dewi, R. K. 2015. Karakterisasi Berbagai Aksesi Sagu (Metroxylon spp.) Di Kabu-paten Sorong Selatan Papua Barat. Tesis. Institut Pertanian Bogor.
Edi Hanzen, W., Sri Hastuti, U., Putera Mak-kadafi, S., Al Asna, P. M., & Sarwendah Asri Nugraheni, F. (n.d.). 2017. Isolasi Dan Identifikasi Bakteri Amilolitik Dari Tanah Yang Tercampur Limbah Kulit Ubi Kayu Di Bondowoso, Jawa Timur.
Fatchiyah, E.L., Arumingtyas S., Widyarti, & Rahayu, S. 2011. Biologi molekuler prin-sip dasar analisis. Jakarta: Penerbit Erlang-ga
Goodfellow, M. 2000. Microbial Systematics: Background and uses In Applied Mi-crobial Systematics (F.G. Priest & M. Goodfellow), eds.) Kluwer Academic Publisher. Dordrecht. Nederlands.
Gurung, N, R Sumanta., Sutapa B., Vivek R. 2013. A Broader View: Microbial En-zymes and Their Relevance in Industries, Medicine, and Beyond. Biomed Research International.
Hariyanto, B. 2011. Manfaat Tanaman Sagu (Metroxylon sp.) Dalam Penyediaan Pan-gan dan Dalam Pengendalian Kualitas Lingkungan. Jurnal Teknik Lingkungan, 12(2): 143-152.
Hanzen, F.W., Hastuti, S.U., Makkadafi, P.S., Nugraheni, A.S.F,. 2017. Isolasi Dan Identifikasi Bakteri Amilolitik Dari Tanah Yang Tercampur Limbah Kulit Ubi Kayu Di Bondowoso, Jawa Timur.
Holt, J. G., Krieg N. R., Peter H. A. S., James T. S., Stanley T. W. 1994. Bergey’s Man-ual of Determinative Bacteriology Ninth Edition. Williams and Wilkins. United States of America.
Jacobs, H., J A. Delcour. 1998. Hydrothermal Modifications of Grabular Starch with Re-tensulasi of Granular Structure. Food Chem, 46(8): 2895-2905.
Kusbandari, A. 2015. Analisis Kualitatif Kandungan Sakarida Dalam Tepung dan Tepung Umbi Gayong (Canna edulis Ker.). Jurnal Pharmaciana, 5 (1): 35-42.
Limbongan, J. 2007. Morfologi Beberapa Jenis Sagu Potensial di Provinsi Papua. Jurnal Penelitian dan Pengembangan, 26 (1): 16-24.
Massora, M., Martani, E., Sugiharto, E., Sar-wom, R, and Sinaga, T. 2017. Biofilm formaton analysis and molecular ident-ficaton of copper-resistant bacteria iso-lated from PT Freeport Indonesia’s tail-ings. Indonesian Journal of Biotechnol-ogy 22(1), 2017, 6–12.
Palmer, T., Bonner P. L. 2007. Enzymes: bio-chemistry, biotechnology, clinical chemis-try.second Edition. Woodhead Publishing. United Kingdom.
Purba, E. 2009. Comparison of hydrolisis of cassava starch (Manihot esculenta) and sweet potato starch (Ipomoea batatas) with cold process using enzyme Acid-fungal Amylase and Glucoamylase. Jour-nal of Science Engineering and Technolo-gy, 2 (2): 7-11.
Puwarni, E.Y., T Purwadaria., M T. Suhartono. 2012. Fermentation RS3 derived from sa-go and rice starch with Clostridium butyr-icum BCC B2571 or Eubacterium rectale DSM 7629. Jurnal Anaerob, 18(1): 55-61.
Rahayu, M. A., Sulistyaningtyas A.R., Darmawati S. 2019. Isolasi Bakteri Hidro-filik Penghasil Enzim Amilase dari Limbah Industri Tapioka. Seminar Nasional Uni-mus. 147-155. (Prosiding).
Risnoyatiningsih, S. 2011. Hidrolisis tepung ubi jalar kuning menjadi glukosa secara enzimatis. Jurnal Teknik Kimia, 5(2): 417-424.
Soeka, Y. S., Rahmansyah M., Sulistiani. 2015. Optimasi Enzim α-Amilase dari Ba-cillus amyloliquefaciens O1 yang Diinduksi Substrat Dedak Tepung dan Kar-boksimetilselulosa. Jurnal Biologi Indone-sia, 11 (2): 259-266.
Sundari, A. S., Purwani N. N., Kurniati A. 2019. Isolasi dan Penentuan Indeks Ami-lolitik Bakteri Dari Sediment Mangrove Di Wonorejo Surabaya. Jurnal Inovasi Pen-didikan Sains, 10 (1): 38-44.
Widjono, A., Mokay Y., Amisnaipa., Lakuy H., Rouw A., Wihyawari P. 2000. Jenis-jenis Sagu Beberapa Daerah Papua. Pusat Penelitian dan Pengembangan Sosial Ekonomi. Bogor.
Wulandari, D., Purwaningsih, D. 2019. Identi-fikasi dan Karakterisasi Bakteri Amilolitik pada Umbi Colocasia esculenta L. Secara Morfologi, Biokimia, dan Molekuler. Jurnal Bioteknologi & Biosains Indonesia, 10 (1): 38-44.
Yuniarti, L. I., G S. Hutomo., A Rahim. 2014. Sintesis dan Karakterisasi Bioplastik Ber-basis Tepung Sagu (Metroxylon sp.). Jurnal Agrotekbis, 2 (1): 38-46.
